抄録
Pyridoxylated hemoglobin (PLP-Hb), a possible substitute for red cells as artificial oxygen carrier, was prepared and characterized physicochemically. The followings are the main points of our analysis.
1) The yield of the pyridoxylation reaction was determined by electrophoresis on cellulose acetate and high performance liquid chromatography (HPLC).
2) Pyridoxylation rate of stroma-free hemoglobin (SFH) from outdated blood was 68.5±4.4% by electrophoresis and 50.5±1.0% by HPLC and significantly higher than that from fresh blood. The pyridoxylation rate of SFH from fresh blood was 59.7±2.9% by electrophoresis and 40.2±1.0% by HPLC.
3) PLP-Hb was separated from unmodified Hb by using ion-exchanger, DEAE Sephadex A-50.
4) There appeared to be two species of PLP-Hb (PLP-Hb-1 and PLP-Hb-2) which could be distinguished each other by electrophoretic charge and P50 value. The P50 value of PLP-Hb-1 and PLP-Hb-2 were 17.5mmHg and 24.5mmHg respectively whereas the P50 value of unmodified Hb was 10.5mmHg.
5) PLP-Hb was stable at 4°C without any significant change of P50 value at least for one month.
