Abstract
A two-channel Auto Analyzer II system (AAII) was tested for routine screening of blood group antibodies in 8, 980 sera during past 18 months. A low ionic strength polybrene method (LISP) was employed to detect IgG antibody.
Manual conventional method was tested in parallel and compared with this method. AAII was not outstanding with respected to detection of some naturally occurring antibodies such as anti-Lewis, anti-P1. For the purpose of preferential detection of antibodies in the P and Lewis systems, we used commercial panel red cells instead of pooled red cells made in our laboratory.
On the contrary, weak immune antibodies missed in the manual method were well detected by AAII. These results suggest that this system is ideally suitable to the detection of IgG antibodies which appears early after transfusion.
we consider that the automated LISP method is very useful in a laboratory engaged in the detection and identification of red cell antibodies.
