1987 年 33 巻 6 号 p. 683-688
Several trials were carried out in 60 cases with intraoperative autotransfusion.
In order to minimize hemolysis and bacterial contamination in red cell suspension which was reconstructed from salvaged blood.
Surface activating agent, poloxamer 188, added in the aspiration fluid, had no protective effect for hemolysis.
The improvement of aspirator, that was able to mix with the aspiration fluid at the top and was able to minimize obstruction by the tissue fragment in the lumen, seemed effective to reduce the hemolysis.
Adding ampicillin sodium of 2g in 1, 000ml of saline for aspiration fluid and furthermore adding either latamoxef sodium of 2g or ceftizoxime sodium 2g in 1, 000ml of saline for the rinsing red cells reduced the bacterial contamination rate from 47.8% to 13.5% significantly.
In addition, it seemed effective to minimize the bacterial contamination rate in this study that double or triple amount of the rinse solution was used in the process constructing red cell suspension and application of negative pressure in the aspiration system was limited strictly only when hemorrhage occurred in the operation field and the aspiration was needed essentially.