Plasma or Cohn fraction (Fr) II+III suspension was mixed with 1/40 volume of HTLV-III solution and processed by the Cohn alcohol fractionation and polyethylene glycol (PEG) fractionation methods.
The spiked HTLV-III can be inactivated by a rate of>104 during the alcohol fractionation step from plasma to Fr-II+III, by>104 from Fr-II+III to Fr-II and by>104 during the PEG fractionation step from Fr-II+III to intravenous IgG (IVIG) fraction.
The cumulative efficiency of the entire process A: Plasma→Fr-II+III→Fr-I→PEG process→IVIG, or B: Plasma→Fr-II+III→PEG process→IVIG is calculated to correspond to an elimination rate of>1012 or>108, respectively.
