イムガードEを用いた白血球除去濃厚赤血球の調製

抄録

Conventional leukocyte removal filters require priming with normal saline, and concentrated red cells have to be diluted with normal saline to give a red cell suspension for speedy filtration. This makes preparation of leukocyte-poor red cells troublesome and time-consumin. The Imugard E filter, in contrast, requires no priming, and allows quick filtration of concentrated red cells with no saline added.
In this study, buffycoat-poor concentrated red cells were filtrated through the Imugard E filter. The leukocytes removed were more than 99%, and no lymphocytes could be detected in leukocyte-poor concentrated red cells. The red cell loss was less than 20%, and the filtration time required was 3-7min.
Direct filtration of concentrated red cells through the Imugard E resulted in no significant chantges in ATP concentration, 2, 3-DPG concentration, and no hemolysis due to filtration was noticed. It may be concluded that the Imugard E is a filter of good performance, simple to use and effective in leukocyte removal.