1989 年 35 巻 6 号 p. 653-658
A quintuple-bag system with a small filter was devised to prepare leukocyte-depleted red blood cells in a closed system. This system is a combination of buffy coat removal by centrifugation and filtration through cotton filter. Approximately 65% of leukocytes were removed in the first step, centrifugation process, and approximately 98% of leukocytes were removed in the following filtration process. Removal of leukocytes increased up to 99% when the filtration was carried out at 4-6°C. Thus, the number of leukocytes remaining in the red cell concentrates derived from 400ml of blood was estimated to be approximately 1.4×107/unit. Therefore, if the red cells prepared by this method are used, the risk of HLA immunization can probably be prevented.