Abstract
Four hundred milliliter of blood was collected from healthy donor in a quadrupl-bag system containing 56ml of citrate-phosphate-dextrose. Whithin 6 hours of collection, the blood was centrifuged at 1, 300×g for 6min in KUBOTA-9810 centrifuge. After centrifugation, the blood was separated using automated separator into platelet rich plasma, buffy coat fraction (approximately 40ml), and concentrated red cells.
Buffy coat fractions contained 72.8±8.4 percent of the leukocytes and 6.1±1.1 percent of the red cells of the original value. Thus, the number of leukocytes remainging in the buffy-coat-depleted red cells were 60±27×107 per unit, in paticular, lymphocytes decreased to 1.9±1.0×107 per unit with removal of 97.1±1.3 percent of the original value. Therefore, this method seems to be useful for preparation of leukocyte poor red cells component.
