日本輸血学会雑誌
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
テラサキプレート (U底) によるウイルス検査凝集法微量化測定の検討
飯島 雅子重田 勝義川田 典子月本 一郎Fumiko Funane
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1990 年 36 巻 6 号 p. 701-704

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A micro-aggregating method using a U-bottom Terasaki Plate was evaluated with blood from 252 patients during the 12-month period from January to December, 1989.
Specimens could be to read with consistency at patient's serum volume and reagent volume of 8-10μl. Correlation of the Terasaki Plate method and the Micro Plate method were shown to be 99% for the PA test (HTLV-I antibody), and 100% for the RPHA (HBs antigen) and PHA tests (HBc antibody).
Antibody titer determination revealed a correlation rate of 82%, without diurnal variation. Determination was possible at a reaction time of 90 minutes; moreover, reagent costs could be reduced to one third.
This method was deemed to be useful for saving serum samples, and was shown to offer reductions in cost and reaction time.

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