A new solid phase system (Capture-R) for the detection of irregular IgG red cell antibodies was evaluated.
In this system, test sera were added to the wells coated with reagent red cells and incubated. After washing plates, anti-IgG coated indicator red cells were added, and the plates centrifuged.
In the case of a positive test, indicator cells adhered to the entire surface of the well. In contrast, in the case of a negative test, the indicator cells formed a compact bottom in the bottom of the well.
Warm type IgG antibodies considered to be clinically important were completely detected by Capture-R, but a part of IgM antibodies such as anti-Lea, anti-Leb, anti-P1 and anti-M were not detected.
We consider that Capture-R is suitable for pre-transfusion tests since it is objective, sensitive and easy to perform compared with conventional antiglobulin test.
