献血者における各種診断薬を用いた抗HCV抗体の検出及びRT-PCR法による遺伝子検出
1992 年 38 巻 1 号 p. 58-63
詳細
1992 年 38 巻 1 号 p. 58-63
The prevalence of antibody to hepatitis C virus (HCV) and HCV RNA were tested in blood doners with ELISA and reverse transcription-polymerase chain reaction (RT-PCR). In this study, four ELISA Kits were used to screen for antibodies to HCV (Ortho-1st:c100-3 viral peptide, TND-2:c-7 and c-11 viral peptide, GOR: host derived peptide and Ortho-2nd: c200 and c22-3 viral peptide). Among 998 doners, the seropositivity rate was 1.2% (Male 1.6%, Female 0.8%) for Ortho-1st, 1.5% (M 1.6%, F 1.4%) for TND-2 and 1.2% (M 1.4%, F 0.8%) for GOR. Also, HCV RNA was detected in 6 samples from 12 positive to Ortho-1st (50.0%), 10 samples from 12 positive to TND-2 (83.3%) and 7 samples from 12 positive to GOR (58.3%).
