PLATELET ACTIVATING FACTOR AND FIBRONECTIN LEVELS BETWEEN THE PERIPHERAL BLOOD OF DONORS AND OF THE BLOOD INTO THE COLLECTED BAGS USING CELL SEPARATOR FOR THE PURPOSE OF APHERESIS

Abstract

Platelets aggregates or floating matters are occasionally observed in the bags of platelet and granulocyte concentrates collected from donors using cell separator. With this in mind, we studied this modality of blood collection with special attentions on donors, platelet activating factor (PAF), fibronectin (FN), blood viscocity and serum protein (albumin/gammaglobulin ratio=A/G ratio) as platelets and granulocytes within the blood collection bags. FN before collection was tended to be higher in donors with higher leukocyte counts. During collection of blood components, both PAF and FN tended as well as blood viscocity and A/G ratio to decreased compared to the baseline level before collection. In the platelet concentrate bags, PAF tended to increase lightly, and FN tended to decrease slightly, compare to those in the circulating blood. Where platelets aggregates or floating matters are observed in the bag, high level of PAF before collection in the donor and also of whose PAF was higher and FN was lower than the baseline values.
The decrease in PAF and FN observed during collection of blood components seemed to be attributable to ACD-A injected during blood collection and to the dilution of blood in the body caused by injection of saline. It was also presumable that platelet activation during extracorporeal circuration could induce platelet aggregation, as evidenced by the increases in PAF and decreases in FN after blood collection and within the collection bags.