ナジョットチェンバー・ハウザーチェンバーを用いた白血球除去血液製剤中の残存白血球数の測定

抄録

We have measured residual leukocytes in leukocyte-depleted blood products using Nageotte and Hausser counting chambers. For testing, one part of platelet concentrate (PC) and red cell concentrate (RCC) were added to 7.5 parts of propidium iodide solution containing RNase, sodium citrate and Triton X-100 or added to 9 parts of Türk's solution containing gentian violet and glacial acetic acid. Stained leukocytes were measured in 2 areas (100μl) with the Nageotte chamber or in 2 areas (6.44μl) with the Hausser chamber. The leukocytes in pre-filtered PC and RCC had first been measured in a Neubauer. hemocytometer and the samples were serially diluted with leukocyte-free PC or RCC prepared by repeated filtration. The number of leukocytes measured with the Nageotte and Hausser chambers as a function of cell number expected from the Neubauer hemocytometer count could be expressed by the regression lines: Y=-3.367+1.199X(r=0.968) for PC and Y=-0.800+0.844X(r=0.906) for RCC. The limit of sensitivity for the counting method using the Nageotte and Hausser chambers was 85cells/ml and 1300cells/ml, respectively. Thus, the hemocytometers, the Nageotte and Hausseri chambers, which have volumes of 50μl and 3.2μl, respectively, and which are greater than that of the Neubauer counting chamber (0.9μl), can provide more sensitive and reliable cell counting than the conventional counting methods such as automated electronic cell counters. The Nageotte and Hausser counting chambers should be useful to count the residual leukocytes in leukocyte-depleted blood products as a standard method for the purpose of quality-controlling them when flow cytometers are not available in blood centers.