Changes of temperature in blood collected with ACD-solution (Formula B) in 250cc bottles were observed by a thermo-couple potentiometer
1) in collection bottles were cooled in an ice-box of 4-6°C prior to the bleeding and in those not previously cooled, and
2) in the collection bottles were immediately immersed in an ice-water pan prior to ice-box storage and in those without such preliminary cooling.
In addition the author also investigated the time during which blood was kept at the temperature above 10°C, and concluded that the cooling effect of immersing the blood bottle in ice-water was striking for rapid cooling while the cooling of the blood bottle prior to the blood collection had little and rather negligible effect.