Out of 3100 separate blood specimens sent to our department about 800 specimens were examined for detection of irregular antibodies. In about 60 cases irregular antibodies were found and specificity of 22 sera were identified, which are summarized as follows:
1) Sera of 10Rh negative female contained anti-D, most of which were produced by isoimmunization during pregnancy. A serum of Rh negative male contained anti-D produced by incompatible blood transfusions.
2) Sera of 4 E-negative female contained anti-E produced presumably by pregnancy. A serum of E-negative contained anti-E produced by incompatible blood transfusions.
3) An example anti-M antibody was found at the time of crossmatching test.
4) In 2 cases of acquired hemolytic anemia both anti-c and anti-E were found.
5) A case of aplastic anemia demonstrated anti-S and anti-Jkb.
6) Two professional AB donors sera contained irregular antibodies anti-A1 and anti-Lea respectively.