Abstract
The relation between color reduction and the number of contaminants was investigated.
For the purpose of the identification of bacteria contaminated in stored blood, a small amount of samples was incubated overnight on culture media. But in practice, it is quite necessary to check the contaminants at the bed side of patients.
We adopted the reduction method of a suitable hydrogen acceptor by bacteria. 2, 6-dichlorophenol indophenol was used as hydrogen acceptor. Glucose, pyruvate, lactate, formate and succinate were used as hydrogen donors, but the reduction was most active when glucose or succinate was used as hydrogen donor. Half ml of the supernatant of stored blood, 0.2ml of 1/10M of phosphate buffer at pH 6.0, 0.2ml of 1/10M glucose and 0.1ml of 0.2mg/ml of 2, 6 dichlorophenol indophenol were mixed and incubated at 37°C. After 30 minutes' incubation, the absorption of 2, 6-dichlorophenol indophenol at the wave length of 605mμ was determined.
