Journal of the Japan Society of Blood Transfusion
Online ISSN : 1883-8383
Print ISSN : 0546-1448
ISSN-L : 0546-1448
Volume 9, Issue 1
Displaying 1-4 of 4 articles from this issue
  • Sotoharu YAMADA, Kumano IZUCHI, Michio ISOMURA, Ryoichi NAITO, Shinich ...
    1962Volume 9Issue 1 Pages 1-15
    Published: 1962
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Normal Dried Human Plasma from a single donor (designated A. H. P.) is produced by The Blood Plasma Corporation of Japan. It contains no preservative and is not treated with ultraviolet radiation, but it is made from a single donor in order to minimize the chance of serum hepatitis. A. H. P., so produced, loses very little of its antihemophilic properties. A. H. P., as well as normal fresh blood, contains 100% AHF and 100% PTC. However, normal dried plasma and bank blood contain only 40% AHF, but they do contain 100% PTC.
    A. H. P. infusion in the treatment of the coagulation defect in hemophilia A, hemophilia B and hemophilia AB produced the same effect as did fresh blood transfusion. The patients' thromboplastic activities were elevated and hemorrhagic symptoms improved.
    Since A. H. P. preserves the antihemophilic properties of whole fresh blood, is easily stored, and a large amount may be given at one time, it is more useful than fresh blood for emergencies.
    It should be emphasized that the measurement of the whole blood clotting time is valueless as a test for evaluating the therapeutic effectiveness of transfusion of fresh blood or infusion of A. H. P. in hemophilia or hemophilia-like states, because the whole blood clotting time is sometimes transiently prolonged after administration of either agent. However, the prothrombin consumption test and the thromboplastin generation test are valuable; as the prothrombin consumption and thromboplastic activity become normal coinciding with a favorable response in the hemorrhagic symptoms.
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  • Kei NAGANO, Toshiko NAKAO, Makoto NAKAO, Toshiko JIMBO
    1962Volume 9Issue 1 Pages 16-17
    Published: 1962
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Human blood was stored for 8 to 9 weeks in polyethylene storing bags, and the effect of addition of adenine or adenine and inosine to the storing ACD medium was investigated.
    When both inosine and adenine were added (AT-blood), normal ATP level was maintained throughout the storage, while the level was falled to as low as one tenth of the normal value when the blood was stored in the usual ACD medium. The addition of only the former compound (A-blood), somewhat protected the ATP level against declination. The effect was, however, much less distinguished than the AT-blood.
    The characteristic discoidal shape was also maintained in the AI-blood.
    Thus, our previous results, obtained from experiments with usual glass storage bottles, were reconfirmed using polyethylene resavoir bags.
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  • Taro TSUCHIYA, Motozi KURATA, Mitsuo YOKOYAMA, Susumu TAKAHASHI
    1962Volume 9Issue 1 Pages 18-24
    Published: 1962
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    Various examples of the R0R0 (-D-/-D-) genotype have been found in England, U. S. A., Japan and Puerto Rico.
    The present paper reports studies of an additional family.
    Characteristics of these bloods as established by previous studies were also seen in our cases, and it seems to reveal a complex array of Rh antibodies.
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  • Masaharu KUROKAWA, Tomoko SANO
    1962Volume 9Issue 1 Pages 25-31
    Published: 1962
    Released on J-STAGE: March 12, 2010
    JOURNAL FREE ACCESS
    The relation between color reduction and the number of contaminants was investigated.
    For the purpose of the identification of bacteria contaminated in stored blood, a small amount of samples was incubated overnight on culture media. But in practice, it is quite necessary to check the contaminants at the bed side of patients.
    We adopted the reduction method of a suitable hydrogen acceptor by bacteria. 2, 6-dichlorophenol indophenol was used as hydrogen acceptor. Glucose, pyruvate, lactate, formate and succinate were used as hydrogen donors, but the reduction was most active when glucose or succinate was used as hydrogen donor. Half ml of the supernatant of stored blood, 0.2ml of 1/10M of phosphate buffer at pH 6.0, 0.2ml of 1/10M glucose and 0.1ml of 0.2mg/ml of 2, 6 dichlorophenol indophenol were mixed and incubated at 37°C. After 30 minutes' incubation, the absorption of 2, 6-dichlorophenol indophenol at the wave length of 605mμ was determined.
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