This study aimed to clarify the effect of untouched-toes on posture stability during a one-legged stance in 201 preschool children (97 boys and 104 girls) aged from 5 to 6 years. Contact surface area of the soles while standing with bare feet was photographed five times per a participant using a Pedoscope. When the toes did not come into contact in at least four of five pictures, a diagnosis of untouched-toes was given. The toes of the supporting leg during one-legged standing tests were used for analysis of the untouched-toe. Posture stability while standing was evaluated using a one-legged standing test while the test subjects’ eyes remained open. One-legged standing time did not differ significantly with respect to the absence, presence or number of untouched-toes. There was no significant relationship between higher/lower groups of onelegged standing time and the rate at which untouched-toes. In addition, a significant difference was not found in the number of untouched-toes between the above both groups. In conclusion, it was clarified that the condition of having untouched-toes in preschool children does not significantly affect posture stability during one-legged stance.