Japanese Journal of Veterinary Research
Online ISSN : 2758-447X
Print ISSN : 0047-1917
Verification of quantitative trait loci located on chromosome 4 that affect mouse behaviors by use of congenic and sub-congenic strains generated from DBA/2 and C57BL/6 mice
Keisuke SatoMasami MorimatsuTakashi Agui
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2023 Volume 71 Issue 2 Pages 48-55


The open field test is a basic test used to assess exploratory behavior, anxiety, and locomotor activity in rodents. Some quantitative trait loci (QTLs) regarding mouse behavior in open field were identified so far. A QTL associated with grooming duration was identified in chromosome (Chr) 4 using recombinant inbred strain made from DBA/2 (D2) and C57BL/6 (B6) mice. We previously generated D2 congenic line, D2.B6-Sen1, in which most region of Chr 4 was replaced to B6 haplotype. In this paper, to confirm the position of this QTL and to investigate candidate genes, two sub-congenic lines, D2.B6-Sen1.1 and D2.B6-Sen1.2, were generated. From results of open field test with a congenic and two sub-congenic strains, we found that D2.B6-Sen1.2 increased grooming duration compared with D2, but D2.B6-Sen1.1 demonstrated opposite result, suggesting two genes responsible for grooming duration located in Chr 4. We also found that only D2.B6-Sen1 mice decreased the time in thigmotaxis zone, suggesting another gene responsible for this behavior located in B6-derived chromosomal region.

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© 2023 Japanese Journal of Veterinary Research Editorial Committee, Faculty of Veterinary Medicine, Hokkaido University
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