Abstract
A quick and efficient method for determining the sex of striped hyena(Hyaena hyaena)was developed by performing Polymerase Chain Reaction(PCR) analysis on sampled DNA. DNA samples were collected from white blood cells and hair roots of striped hyenas. PCR was performed using the primers to amplify the sex-determining region Y(SRY). In the test, a DNA band that can be regarded as the target DNA of 214 bp SRY sequence was detected only in males' samples. Therefore, in the case of the striped hyena, as well as other mammalian species, the SRY conserved region is specific only to male. A detection sensitivity test was carried out. The target region with 100 cells or with a single hair root was able to be detected. We conclude that this method, with high sensitivity and easy procedure, seems to present an easy and efficient way for future sex determination in striped hyena.
