Abstract
The world is now in the midst of the fastest mass extinction of wildlife. The author believes that preserving the threatened species is the urgency of the mankind. In the promotion of the ex-situ conservation, artificial reproductive technologies, such as artificial insemination, in vitro fertilization including intracytoplasmic sperm injection, embryo transfer, cryopreservation of germplasm, in vitro spermatogenesis, nuclear transfer and possibly cloning, become useful tools. In this review, I describe various techniques for collecting the germplasm from wild animals (artificial vagina method, manual stimulation, electroejaculation, postmortem recovery, etc.). Finally, the future of our planet depends on human beings and animals learning to live together.
