Paternity Determination of Captive Southern Pudu (Pudu puda) Using Bovine and Ovine Microsatellite Markers

Abstract

　In the current study, we attempted to determine the paternity of captive southern pudu (Pudu puda) using bovine and ovine microsatellite markers. Of 15 markers tested, nine (60.0%) were successfully amplified and seven were polymorphic, with the number of alleles per locus ranging from three to five. Observed heterozygosity in the seven markers ranged from 0.667 to 1.00. Most of these polymorphic markers could be used to accurately verify the relationship between father and offspring. These bovine and ovine microsatellite markers may be useful for parentage testing and genetic diversity analysis in southern pudu.