Studies on the Lysis-Phenomenon of Leucocytes
Rep. II. Observations on the Sensitized Rabbits by Human Serum
1959 Volume 11 Issue 2 Pages 164-171
Details
1959 Volume 11 Issue 2 Pages 164-171
The rabbits were sensitized by 1.0cc of human serum given intravenously every other day three times. Two weeks after sensitization, precipitation titer of rabbit serum was quantitatively measured in vitro and morphologically observed under phase-contrastmicroscope.
Furtherly, existence of antigen specificity was investigated by comparing the cases added with human serum to those added with ovo-albumin to the human-serum-sensitized rabbits.
1) When corresponding antigen was added to the peripheral blood of human-serumrabbit in vitro, rate of leucocytic decrease was recognized to be increased, morphologically lysed, degenerated cells were increased in comparison with those of pre-sensitization.
2) Neutrophiles acted main part of role in the phenomenon of leucolysis, each neutrophile presented difference in reaction, so that there recognized various stages from nonaffected ones to lysed ones.
3) The cells to meet lysis presented distinct Brown movement of granules, and swelling of nucleus and cytoplasm, then destruction of cytoplasmic membrane was followed with the destruction of nucleic membrane. Such a course was completed mostly in a few minutes, and in ten to fifteen minutes at the latest.
In the degenerating cells, there is also the difference of reaction, and required time is not constant, because of their grade of reaction.
4) No certain parallel relationship could be recognized between precipitating titer and the phenomenon of leucolysis.
5) In this experiment, antigen specificity was also recognized.
