Unexpected Antibodies Detected in Our Blood Transfusion Unit during Four Years

Abstract

The status of the unexpected antibodies detected in blood donors or recipients and in the newborns with haemolytic diseases, using physiological saline test, high protein test, bromelin test and anti-globulin test in our blood transfusion unit were reported.
1.226 samples out of 12,875 samples were positive for unexpected antibodies. Anti-D, -E, -Le^a, -Le^b and -P₁ antibody were predominant among the antibodies.
2. It should be noteworthy that about 40% of anti-Le^a or Le^b antibodies, which may cause hemolytic diseases, were detectable only by anti-globulin test.
3. Among warm-type auto-antibodies showed Rh specific ity (anti-C, anti-E, anti-D antibody).
4. Among 160 samples from incompatible pregnancies,21 gave positive results: i. e.15concerning Rh system, and 6 ABO system.
5. Exchange transfusion were car ried out in 10 cases due to hemolysis caused by blood type incompatibilities: The antibodies detected were 1 anti-D,1 anti-D+ E,3 anti-E,2 anti-E-F c, as well as 1 anti-A and 2 anti-B. These results suggest the importance of E antibody, not only D antibody, for transfusion and pregnancy.