Immunobiological Study on the Complement of the Tumor-bearing Rats
Part I. The Behavior of Complement in the Tumor-bearing Animals
1983 Volume 35 Issue 3 Pages 484-499
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1983 Volume 35 Issue 3 Pages 484-499
An experimental model of the tumor-bearing hosts was prepared by inoculating cells of Walker carcinosarcoma 256 subcutaneously into Wistar-JCL rats. Their total hemolytic complement activity (CH50), relative amount of complement component C3 (C3), hemolytic titration of C1-C2 (C142 titration) and hemolytic activity of C3-C9 (C3 total) were determined as parameters of complement response in hosts.
Next, the tumor-bearing rats were s ubjected to surgery 3 days after inoculation and divided into groups of curative resection and non-curative resection of tumors. CHSO was titrated in both groups and the following results were obtained.
1. A burned group of rats, experimentaly induced inflammation showed significantly higher complement activity than the tumor-bearing rats (P<0.001).
2. A high correlation was noted between CHSO and C3 value in the tumor bearing rats (y=0.71x + 44.23, r=0.727, P<0.001).
3. The changes of CHSO in relation to tumor volume showed significantly higher values in tumor-bearer below 25 cm3, but significantly lower values above 201 cm3 than the value of control (P<0.001). The same tendency was noted in C3 total, while no remark a ble change in C142 titration (not significant).
4. The change of CHSO in relation to the interval after tumor inoculation showed the maximum levels on the 2nd day (P<0.001), but significantly decreased on the 14th day (P<0.001). The same tendency was noted in C3 total, while no remarkable change in C 142titration (N. S).
5. In the group of curative resection of tumors, high complement activity was maintained until the 28th postoperative day. While in the group of non-curative resection o f tumors, complement activity was noted until the 14th postoperative day.
From these results, complement response to the inoculated tumor cells was noted in the early stadium after the inoculation, via properdin system (i. e.; alternative pathway). High complement activity was noted in the group of complete surgical removal of tumors for a long postoperative period. This fact seems to suggest the usefulness of complement as a parameter of hosts'immunological response to the tumor. In addition this study confirms the effectiveness of curative surgery on tumors.
