Modern Medical Research to "OKETSU" State

Studies in Microcirculation and Hemorhology

Abstract

SUBJECTS AND METHODS
In this report,56 patients in Oketsu state (24 of male and 32 of female) who were applicated to Keishi-bukuryo-gan, Toki-syakuyakuTsan were examined. In addition,24 patients (8 of male and 16 of male) who were applicated to Oren-gedoku-to were also examined. Ten patients of Oketsu state and 10 of Non-Oketsu state were examined before and after administration of Kanbaku-taiso-to.
Whole blood viscosities were measured at high shear rate (94.5 sec-1) and at low shear rate (0.376sec-1) by CONTRAVES Low Shear 30. R In order to know the erythrocyte deformab ilities, the passage times of 40% red blood cell suspensions were measured. Plasma passage time, peripheral blood changes (WBC, RBC, Hb, Ht, MCV, MCH, MCHC) and biochemical changes (TP, Alb, yGlb, ATIII, Fib) were also measured. Blood samples were withdrawn from elbow vein at before and 60 min. after administration of each drug of 5.0 grams.
An Intravital Videomicroscopic System (IVVMS) develop ed by Tsushima was used to observe and record the microcirculation kinetics and on the human bulbar conjunctiva. A Distance Meter Method developed by Tsushima was utilized for measurement of, the blood flow velocity.
Diameters of erythrocyte-colums were used as the internal diameters of blood vessels, excluding plasma sheath. Blood flow volumes were calculated by multiphying cross-sectional area and average blood flow velocity on the assumption that the vessel cross-section is circular.
Microcirculation was observed and measured at the same part of the same vessel before and 60 min. after administrations of drugs.
For the evaluation of "Oketsu" state, we used "Oketsu" score established by Terasawa.
All results are expressed as mean ± S. E., the significance is assessed using the pairedt test, the level of significance being calculated at p<0.05.