1999 Volume 51 Issue 2-4 Pages 125-131
To investigatet he effecto f uremiaa nd hemodialysios n hem oglobignl ycosylationan d carbamylation, (1) pre- and post-dialysise rythrocytes(RBC) were incubatedw ith variousc onditionsa nd their HbAi changesw ere measureda, nd (2) wholeb loodw as incubatedw ith glucoseo r urea, followedb y measuring HbA1c oncentrationasf ter separatingold and youngR BCb ymeanso fpartial hemolysisS. tudy subjects were 25 patientsw ith chronicr enal failure on hemodialysisa, n d H bA1a nd its fractionsw ere measured by microchromatographteicc hniquesH. bAi, A ia-f-ba nd A le did not changes ignificantlyb y a session of hemodialysios f 4-5 hrs. Howeverp, re-dialysisb loods howeda significanth igherH bA1a nd its fractions than post-dialysisb loodw henm easureda fter incubatingt hem for 7 days. H bAi and its fractionss howed an increasei n a greater degreew hen incubatingR BCw ith pre-dialysisp lasmat han with post-dialysis plasma. R BCf rom patientsr eceivingh emodialysiws ith glucose-containindgia lysates howeda greater increase in H bA1b y the incubationY. oungerR BCw hich are morer esistant to low osmoticp ressure than old RBCw ere apt to be glycosylateodr to be carbamylated. These findingss uggestt hat uremiaa nd g lucose-containinhge modialysatea ccelerateR BCg lycosylation and carbamylationw hich might relate to RBCm etabolisma nd that the sufficienth emodialysis is necessary to prevent Hb abnormal metabolism.
