Abstract
The aim of this study is to induce effective superovulation in Japanese Black cows with hMG and to produce high-quality embryos in a short interval of superovulation treatment. In the first superovulation treatment, a total of 750 IU hMG was used and after collection, 50 ml of iodine was infused into the uterus and a 25 ml PGF2α intramuscular injection was also given. After confirming the return of oestrous, a second treatment was conducted with a total of 600 IU hMG. Although the total number of collected ova/embryos and transferable embryos was less in the second treatment than in the first one, the ratio of transferable and excellent embryos was considered to be high. In conclusion, it was indicated that 600 IU hMG is effective for superovulation and that iodine preparation and PGF2 α produce high-quality embryos in a short interval of superovulation treatment.
