キメララットの作出
1985 年 2 巻 2 号 p. 123-128
詳細
1985 年 2 巻 2 号 p. 123-128
Mammalian chimaeras are used for reserch on embryology and interaction of distinctive cells in genetic disorder. Though chimaeric mice have been produced on a large scale, there are a few reports concerning chimaeric rats. The present study was attempted to produce chimaeric rats by aggregation between Wistar and Hybrid (Wistar x Brown Norway) embryos. The results obtained are summarised as follow.
1. Aggregated embryos cultured in modified medium (Dulbecco's PBS (-) with Whitten's nutrient saline) or Ham's F-12 with 5% fetal calf serum developed to blastocyst in higher proportion compared with modified medium with 10% bovine serum albumin.
2. A total of 185 aggregated embryos was transfered and eighteen of them developed to chimaeric rats. Fifteen of the chimaeric rats grew up to adult.
3. Sex ratio of chimaeric rats was 2: 1.
4. All of the chimaeric rats had reproductive ability.
