Many kinds of culture medium are used for human IVF-ET at various institutions around the world, but the reason for their use is not always clear.
We examined such culture mediums for human IVF as Hoppe & Pitts, mEarles, Ham's F-10, T6 and for animal IVF as mBWW, mKRB, TALP-2 for their applicability to fertilization and to embryo culture by the hamster test and cultural experiment of mouse 2-cell stage embryo, respectively. The mBWW showed the most favorable results in both experiments. It seems important for the mBWW to be reconsidered as a culture medium for human IVF.
