仔雌牛の卵胞卵を用いた体外受精
1987 年 4 巻 1 号 p. 9-15
詳細
1987 年 4 巻 1 号 p. 9-15
In an attempt at collection of an increased amount of follicular ova, superovulation was induced in prepuberal, sexually immature calves (8-12 weeks old) by intramuscular injection of 1500 iu of PMSG followed 5 days later by intravenous injection of 1500 iu of hCG. The ovaries were removed by laparotomy at 24 hours after hCG treatment. The ovaries from one animal contained 70 follicles above 10 mm in diameter, 13 follicles 9-5 mm and 145 follicles above 2mm. The ovaries from the other animal contained 86 follcles'measuring more than 2 mm in diameter. The in vitro fertilization exper-iment using the collected follicular oocytes resulted in 58.2% sperm penetration and 44.9% fertilization with female and male pronuclear formation. The results suggested that ovaries from prepuberal cattle have a hicrher Potentiality for ova.
