マウスの受精、胚発生および胚盤胞ハッチングに及ぼすインドメサシンの影響

抄録

The effect of indomethacin (1M), an inhibitor of prostaglandin (PG) synthesis, on fertilization, early development and hatching of blastocysts was examined using ICR mouse eggs.
(1) When eggs obtained from the oviducts of superovulated females 15 hours after hCG injection were denuded with hyaluronidase and cultured for 2 hours in a modified Krebs-Ringer bicarbonate solution containing 100nM, 1μM, 10μM, 100μM or 200μM of 1M before being inseminated iv vitro, 55.9%, 50.0%, 47.1%, 50.0% and 47.5% of the eggs were fertilized respectively, showing no significant differences from that obtained in an 1M-free (control) medium (55.8%). Fertilization rate was significantly lowered only in the highest (400μM) concentration of 1M.
(2) When 2-cell embryos were cultured for 48 hours in the presence of 100nM, 1μM. 10μm or 100μM of 1M, 79.6%, 81.0%, 81.1% and 85.7% of the embryos respectively were developed into blastocysts, but the percentages showed significant decrease in higher concentrations (200μM) of 1M and no blastocysts were observed in a medium containing 400μM of 1M.
(3) The percentages of hatched blastocysts were significantly decreased when blastocysts obtained 96 hours after hCG were cultured with graded concentrations of 1M higher than 1μM, resulting in only 6.7% of hatched biastocvsts in a medium containlng 400μM of 1M.
These results suggest that PG synthesis plays an important role in early development, especially in the hatching of blastocyst.