Abstract
Cellulose pyrolysate containing levoglucosan (1, 6-anhydro-β-D-glucopyranose) was hydrolyzed to glucose with 0.2 mol/l H2SO4 at 121°C for 20 minute. Such glucose in hydrolysate mixture was treated by neutralization with Ca(OH)2 and adsorption with diatomite, and then was fermented to ethanol by S. cerevisiae and P sp. YZ-1. The ethanol of 0.45 g/g glucose was obtained with initial glucose of 41.9 g/l by S. cerevisiae. Furthermore, by training culture of S. cerevisiae 12 times in the hydrolysate medium containing 60.0 g/l glucose, the trained strain S. cerevisiae (R) could use 95.0% glucose and give 40.2 g/l ethanol in the fermentation with the hydrolysate medium containing 95.8 g/l glucose.
