牛精子の生存率と運動性に及ぼす弱い定常磁場の影響

抄録

The present paper deals with the effect of a static magnetic field on the viability, motility, and velocity of thawed cattle sperm collected from four bulls and stored in straws. Each sample was exposed continuously for 0, 1, 2, 4, 6, or 8 hours to magnetic field of 1000 Gauss at 4°C, 20°C, or 38°C. A solution of 2% Eosin Bluish was used to determine the sperm's viability. The motility and velocity were determined by means of the CellSoft computer-assisted sperm motion analysis system (CASA). The viability of sperm exposed to the magnetic field was higher than that of non-exposed sperm at 4°C (8 hours), 20°C (6 and 8 hours), and 38°C (2 and 4 hours). Magnetic field exposure at 38°C for one hour also enhanced the motility of the sperm. However no significant differences were found in the velocity of sperm as a result of the treatment. These findings suggest that the effectiveness of the magnetic field is high for sperm viability, but only slight for motility, and negligible for velocity.