Abstract
Recently, it was shown that the analysis of surface charge in peripheral lymphocytes by electrophoresis provides the information concerning immunological states of cancer patients We applied this method to the pre-operative urogenital cancer patients or the patients who had been post-operatively receiving Krestine (PSK) and CQ, or Krestine (PSK) and Honvan (Prostatic cancer).
Of 69 subjects examined, 45 were cancer patients (13 with bladder cancer, 10 with renal cell carcinoma, 7 with testicular tumor, 11 with prostatic cancer, 2 with ureteric cancer and 2 with uterine cancer) and 24 normal subjects. Lymphocytes were isolated from heparinized blood by the gradient method of Boyum by using Ficoll-Hypaque. The electrophoretic mobility of lymphocytes was determined with a fully automated cell electrophoretic apparatus (Parmoquant : produced by Kureha Chemical Industry Co., Ltd., Tokyo, Japan). Electrophoresis was carried out at 13 mA for 3 s at 24°C. Ten to 20 migrating cells in the microscopic field were momentarily recorded by image processing and the data were stored in a computor. One hundred cells were measured in one sample. The measurements, e.g. mean mobility of all cells, standard deviation and their histograms, were printed out.
