抄録
We selected a subclone of HeLa-S3 cells to depict the relationship between cellular adherence to the extracellular matrix and pathological features of tumors derived from these cells. The selected subclone (PA cells) adheres poorly to type-IV collagen as compared to the wild type cells (EA cells) which attach easily to type-IV collagen. In vitro, it was confirmed that PA cells adhered poorly to type-IV collagen even after a long term of culture and showed weaker chemotactic activity toward this BM component. Injected into nude mice, PA cells formed smaller tumors than EA cells.
Histologically, both derived tumors showed construction of basement membrane-like scaffolds that stained positively for type-IV collagen and laminin. EA cell tumors demonstrated frequent absence of and thinning and gaps in their basement membrane, which shows the invasive activity of the tumors.
PA cell tumors showed a more regular basement membrane with predominent desmoplasia.
