Abstract
Therapeutic drug monitoring（TDM）that can be performed near the patient is helpful for safe and effective pharmacotherapy. We aim to realize rapid TDM that does not require blood sampling and we investigate the potential of Raman spectroscopy of tear fluid with paper substrates. For this purpose, it should be shown that Raman spectroscopy can identify drugs in human tear fluid, and it is enough sensitive for TDM, and Raman spectroscopy with paper substrates is available for drug identification. In this study, we revealed that Raman spectroscopy could identify the therapeutic drug in artificial tears on silicon coated glass substrates, the sensitivity was enough to detect the drug in aqueous solution whose concentration was equivalent to real human tear fluid, and the drug absorbed in paper substrates was detectable by separating the spectra of drug from that of paper substrates.
