Vitamin Levels in Lung Tissue of Rats with Bleomycin Induced Pulmonary Fibrosis

Handan MERT; Ibrahim YORUK; Ali ERTEKIN; Semiha DEDE; Yeter DEGER; Fatmagul YUR; Nihat MERT

doi:10.3177/jnsv.55.186

Note

Vitamin Levels in Lung Tissue of Rats with Bleomycin Induced Pulmonary Fibrosis

Handan MERT ^†, Ibrahim YORUK ^†, Ali ERTEKIN ^†, Semiha DEDE ^†, Yeter DEGER ^†, Fatmagul YUR ^†, Nihat MERT ^†

Author information

Handan MERT ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Ibrahim YORUK ^†
Department of Chemistry, Faculty of Art and Science, University of Yuzuncu Yil
Ali ERTEKIN ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Semiha DEDE ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Yeter DEGER ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Fatmagul YUR ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Nihat MERT ^†
Department of Biochemistry, Faculty of Veterinary Medicine, University of Yuzuncu Yil
Author contributions

†These authors contributed equally: Handan MERT, Ibrahim YORUK, Ali ERTEKIN, Semiha DEDE, Yeter DEGER, Fatmagul YUR, Nihat MERT

Keywords: bleomycin, pulmonary fibrosis, vitamins

JOURNAL FREE ACCESS

2009 Volume 55 Issue 2 Pages 186-190

DOI https://doi.org/10.3177/jnsv.55.186

Details

Abstract

Bleomycin (BLM) is a chemotherapeutic agent against different carcinomas, one dose of which causes dependent pulmonary fibrosis. The present study was taken up in order to measure the retinyl ester, α-tocopherol and cholecalciferol (vitamin D₃) level in lung tissue in the rats following BLM-induced fibrosis. Fourteen rats were randomly divided into two groups as a control and a BLM group. On the day of the experiment, the BLM group rats were instilled with BLM (7.5 mg/kg) and the control group with sterile saline intratracheally. Fourteen days after instillation, rats in each group were sacrificed and the lungs were prepared for histopathological examination and determination of the vitamin levels with a HPLC system. The levels of retinyl ester, α-tocopherol and vitamin D₃ in the lungs of the BLM group were determined to be lower than in the controls. There was statistically significant difference for the α-tocopherol and vitamin D₃ concentrations compared to the control group (p<0.01, p<0.001), respectively. According to these results in pulmonary fibrosis, vitamins were consumed by the lung tissue and their levels decreased.

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