2010 Volume 56 Issue 1 Pages 9-12
Thiamin (vitamin B1) is known to activate carbohydrate metabolism in part through activation of pyruvate dehydrogenase. The purpose of this study was to investigate the effect of thiamin tetrahydrofurfuryl disulfide (TTFD), a thiamin derivative, on utilization of exogenous glucose by measuring oxidation of 13C-glucose at rest and during prolonged exercise in mice under normal dietary conditions. Mice orally ingested TTFD (0.1 mg/g BW [body weight]) and 13C-glucose (0.8 mg/g BW) or 13C-lactate (0.1 mg/g BW) plus glucose (0.8 mg/g BW) at rest or before endurance running. The average percent of 13C atoms in total 12C+13C (13C atom%) in expired air after ingestion of 13C-glucose at rest was significantly lower in the TTFD group than in the control group. No significant difference was found in 13C atom% in expired air after ingestion of 13C-glucose and prolonged exercise. In addition, no significant effect of TTFD was found in expired 13C atom% after ingestion of 13C-lactate plus glucose at rest. TTFD also had no effect on concentrations of muscle or liver glycogen at rest. These results suggest that TTFD, which is a thiamin derivative, decreases oxidation of exogenous glucose at rest, but not during exercise.