Time Reduction of Vitamin B₆ and Inositol Assay by Using Lyophilized Saccharomyces cerevisiae ATCC 9080

Abstract

The purpose of this study was to improve the efficiency of the microbiological assays for vitamin B₆ and inositol by using lyophilized cells from Saccharomyces cerevisiae ATCC 9080. The use of lyophilized cells as an inoculum was assessed to avoid time-consuming processes like cell precultivation and washing. The authors also examined the effects of various protectants such as skim milk, lactose, maltose, and trehalose on the assay. The viable cell counts of the lyophilized cells were approximately equal. The standard curves for vitamin B₆ and inositol concentrations obtained using lyophilized cells with maltose and intact cells gave similar linear ranges. Furthermore, the measured vitamin concentrations of the standard reference material 1849 were in the range of the established values. Therefore, lyophilized cells with maltose are potential alternative inocula for the turbidimetric method. This will increase the overall convenience associated with microbiological assays.