Effects of Vitamin E Supplementation on Antioxidant, Inflammatory Biomarker, and Cell Viability of Peripheral Blood Mononuclear Cells from Japanese Black Calves with or without Lipopolysaccharide Stimulation

Abstract

The purpose of this study was to determine the effects of vitamin E supplementation on antioxidant, inflammatory status, and cell viability of peripheral blood mononuclear cells. Eighteen clinically healthy Japanese Black calves were used in this study. The peripheral blood mononuclear cells (PBMCs) were isolated from their venous blood. PBMCs were cultured with vitamin E (vitamin E group) or without vitamin E (control group), and stimulated with or without lipopolysaccharide (LPS). As a result, the total antioxidant capacities, which are the reducing power of components from Fe³⁺ to Fe ²⁺, in the cell culture supernatant with or without LPS stimulation were significantly higher in the vitamin E group than that in the control group (p<0.05 or p<0.01, respectively). Tumor necrosis factor alpha in the cell culture supernatant with LPS stimulation was significantly lower in the vitamin E group than in the control group (p<0.01). The viability of cells cultured with LPS stimulation was significantly higher in the vitamin E group than in the control group (p<0.05). These results suggested that vitamin E might be related to the antioxidant, anti-inflammatory, and cell viability of PBMCs obtained from calves.