Abstract
From the comparison of the values of twenty-two long stored samples before and after alumina column chromatography, the following results were obtained.
1. Nine samples (two vitamin preparations and seven fish liver oils) were decided to be in need of purification by chromatography.
2. However, the increase in vitamin A value was very small. When these observations and the complicated procedure of alumina column chromatography were considered, it might be concluded that the application of chromatography was not always necessary for vitamin A determination of these samples.
3. The Brunius regulation might be available to decide the necessity of chromatographic purification.
