Abstract
1. The present authors have attempted to elucidate whether there may be possible occurrence of water-soluble vitamin D sulfate in fresh mammalian milk.
2. Water-soluble vitamin D sulfate was isolated as its water-insoluble barium salt from the aqueous layer of fresh deproteinized milk.
3. After repeating careful analyses, about 204I.U. of vitamin D was shown to be present in 1 liter of fresh cow's milk in a water-soluble form, whereas about 36I.U. per liter in a lipid-soluble form. In case of fresh human milk, it was estimated to be about 950I.U. per liter in a water-soluble form while 15.7I.U. in a lipid-soluble form.
