Effect of Seizure Induced by Hydroxymethylpyrimidine on the Synthesis of Rat Brain Amino Acids from Glucose-U-¹⁴C

Abstract

The free amino acids and glucose of rat brains were determined after separation by high-voltage paper electrophoresis and the isotopic incorporation from glucose-U-¹⁴C into the individual amino acids was measured at the preconvulsive stage induced by hydroxy-methylpyrimidine.
The absolute level of each amino acid was not so decisively varied, but the incorporation of labeled ¹⁴C-glucose into the amino acids was considerably lowered in the drug-treated group.
The change of incorporation among each amino acid was relatively constant. It is assumed that the metabolic disturbances of glucose were widely brought about in the drug-treated rats.
These changes were not restored by the concomitant administration of pyridoxal-HCI, though the convulsion was completely protected.