ISOLATION OF THE THIAMINE DESTRUCTION PRODUCTS BY THE THIAMINASE OF BACILLUS ANEURINOLYTICUS

Abstract

The destruction products of thiamine by B. aneurinolyticus were isolated in a crystalline form; 4 methyl 5 β hydroxyethylthiazole as picrate in the yield of 63per cent and 2 methyl-4-amino-5-hydroxymethylpyrimidine in a free base in the yield of 41per cent. The latter was further identified as 2-methyl 4 amino 5-hydroxymethylpyrimidine hydrochloride.