QUANTITATIVE DETERMINATION OF PYRIDOXALPHOSPHATE BY APOTRYPTOPHANASE OF ESCHERICHIA COLI

Abstract

1 A simple method for the determination of PALP was devised using the apotryptophanase of Escherichia coli which contains neither pyridoxalkinase nor PALP-decomposing enzyme. The limits for measurement of PALP are roughly 0.03 and 0.3γ, and PAL, PIN, PAM and ATP did not show any disturbing effects on this method.
2. The PALP levels of human serum were measured, and followed after intravenous administration of PALP, PAL or PIN.
3. The PALP levels of the organs of normal and thyreoidectomized rabbits were measured.