Abstract
Prolonged incubation of maltose and pyridoxic acid with enzyme pre-paration from rat liver gave a new compound together with 5'-O-(α-D-glucopyranosyl)-4-pyridoxic acid. The purified compound was obtained as a colorless powder from the incubation mixture by isolation procedures consisting of charcoal treatment and column chromatography on DEAE-cellulose and Sephadex G-10. The compound, which was identified as 5'-O-(α-D-maltosyl)-4-pyridoxic acid, was produced through successive transfers of glucosyl groups from maltose to pyridoxic acid, (l→4) bond being formed.
