Journal of Nutritional Science and Vitaminology
Online ISSN : 1881-7742
Print ISSN : 0301-4800
ISSN-L : 0301-4800
THE INTRACELLULAR LOCALIZATION AND STABILITY OF THE DIHYDROFOLATE SYNTHETASE IN PEA SEEDLINGS
Masamichi IKEDAKazuo IWAI
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1975 Volume 21 Issue 1 Pages 1-6

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Abstract

The amount of folate compounds rapidly increased during germination of the pea. An especially high increase was observed 2 days after sowing, with a continuously increasing content during germination. Dihydrofolate synthetase [EC 6.3.2.12] activity also increased with the in-crease in the amount of folate compounds, and the maximum activity was observed at 6 days after sowing in the dark and at 8 days after sowing in the light. The dhydrofolate synthetase was localized mostly in the mitochondrial fraction and it was easily extracted from the cell particles by osmotic shock. A similar localization was also observed in spinach leaves. The enzyme which was extracted from the isolated mitochondria was relatively stable in comparison with that extracted from whole cells.

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