Separative Determination of Ascorbic Acid and Erythorbic Acid in Animal Tissues by High-Performance Liquid Chromatography

Abstract

A method for the separation and determination of ascorbic acid (AsA) and erythorbic acid (ErA) in animal tissues is described. It employs high-performance liquid chromatography of a LiChrosorb-NH₂ column in conjunction with a mixture of acetonitrile, acetic acid and water as an eluant. Application of the method, which is sensitive, rapid and simple, to the analyses of AsA and ErA in animal tissues such as liver, adrenals, spleen, kidneys and heart gave satisfactory results. Dehydro AsA and dehydro-ErA in rat liver could be determined after reduction with H₂S. The method was shown to be useful for the routine analyses.