High-Performance Liquid Chromatographic Method for the Analysis of Nτ-Methylhistidine in Food, Chicken Excreta, and Rat Urine

Abstract

A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and postcolumn fluorescence derivatization for the analysis of Nτ-methylhistidine in food, chicken excreta, and rat urine is described. Nτ-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The Nτ-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15mM sodium octane sulfonate in 20mM potassium phosphate), and subjected to high-performance liquid chromatography.