Changes in Glucose Uptake by and Phlorizin Binding to Brush-Border Membrane Vesicles of Small Intestine from Streptozotocin-Induced Diabetic Rats

Abstract

The changes in the intestinal glucose transport in strep-tozotocin-induced diabetic rats have been demonstrated with brush-border membrane vesicles. When Na⁺ -dependent D-glucose uptake and phlorizin binding activities were compared, both significantly increased either in the jejunum or ileum of diabetic rats compared with those of control. Kinetic studies showed the increases in the V_max of glucose transport and the maximum binding of phlorizin (B_max), whereas the K_t. and K_d remained unchanged, respectively. These results suggested that the increase in glucose transport in diabetic rat intestine was due to the increase in the number of the intact glucose transporters.